Revised 2008/05/22.
The most important parts of a chapter on cofactors and vitamins are the detailed mechanisms of action of these molecules. Learning the mechanism of the NAD+ reaction, for example, will mean that you know the most difficult part of the mechanisms of the many enzyme-catalyzed reactions that involve the nicotinamide ring. This holds true for all the cofators discussed in this chapter.
In this chapter, you will make heavy use of your knowledge of structure (Lewis diagrams) and geometry (VSEPR theory)
In this chapter, you will make heavy use of your electron-pushing skills, and of your understanding of bonding theory (hybridization, aromaticity), and reactivity (nucleophiles, electrophiles, acids, bases).
Memorize basic electron pushing for one representative reaction involving each of the following cofactors: ATP, NAD+, FAD, and lipoic acid. Make sure you can follow and understand (but not necessarily memorize) mechanisms involving TPP, pyridoxal phosphate, and biotin. Place your emphasis on the chemical changes within the cofactors themselves. Memorizing structures is not as important and being able to push electrons in structures that are provided.
Come to class prepared to write a mechanism for a simple reaction involving one of these cofactors: ATP, NAD+, FAD, or lipoic acid. Structures of cofactors provided.